985 Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation

Head and neck dermatitis (HND) is a refractory phenotype of atopic (AD) can be therapeutic challenge. This study aimed to determine the link between HND fungiome examine contribution Malassezia furfur. To elucidate mechanism underlying effects M. furfur, human keratinocytes dermal endothelial cells were cultured with The downstream various cytokines, including inflammation angiogenesis, investigated by real-time quantitative PCR. identify association changes in lipid composition furfur sensitization status, D-squame tape stripping was performed. Lipid evaluated focusing on ceramide species using liquid chromatography coupled tandem mass spectrometry. IL-4 treated produced significantly more VEGF, VEGFR, IL-31, IL-33. Stratum corneum analysis revealed decreased levels esterified omega-hydroxyacyl-sphingosine, indicating skin barrier dysfunction HND. Finally, growth inhibited addition these ceramides culture media, while other microbiota, Cutibacterium acnes, not inhibited. As result, under AD patients HND, would proliferate, which may enhance pro-inflammatory cytokine levels, tissue remodeling. Thus, it plays central role pathogenesis AD.